539569

PP2C, α-Isoform, His•Tag^®, Human, Recombinant, E. coli

• Synonym(e): PPM1A, Protein Phosphatase 2Cα
• UNSPSC-Code: 12352202

Eigenschaften

• Assay: ≥95% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥8,000 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Farbe: clear colorless
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human PP2Cα fused to a His•Tag sequence and expressed in E. coli. A Mn²⁺- or Mg²⁺-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response, gene expression and replication in eukaryotes. Recently it has been shown that PP2Cα inhibits cell growth and activates the p53 pathway.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 37°C, pH 7.4 using 10 mM of substrate.

Physikalische Form

In PBS, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Ofek, P., et al. 2003. J. Biol. Chem.278, 14299.
Das, A.K., et al. 1996. EMBO J.15, 6798.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable