524625
Phosphatase Inhibitor Cocktail Set II
A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.
Synonym(e):
Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352200
NACRES:
NA.77
Empfohlene Produkte
Qualitätsniveau
Form
liquid
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
Versandbedingung
wet ice
Lagertemp.
−20°C
Verwandte Kategorien
Allgemeine Beschreibung
A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.
Anwendung
Phosphatase Inhibitor Cocktail Set II has been used:
- in triple detergent buffer to lyse cultured cells to extract total protein for western blots
- as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins
- as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2)
- as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting
Biochem./physiol. Wirkung
Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.
Leistungsmerkmale und Vorteile
- Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
- Economical: ready-to-use format minimizes wastage.
- Effective: one ml is adequate for approximately 100 ml extraction buffer.
- Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).
Komponenten
Provided as a set of five 1 ml vials containing the following components:
- Imidazole (200 mM)
- Sodium Fluoride (100 mM)
- Sodium Molybdate (115 mM)
- Sodium Orthovanadate (100 mM)
- Sodium Tartrate Dihydrate (400 mM)
Warnhinweis
Toxicity: Standard Handling (A)
Sonstige Hinweise
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Lagerklassenschlüssel
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
EU REACH Annex XVII (Restriction List)
CAS No.
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Rui Dong et al.
Journal of neuroinflammation, 19(1), 64-64 (2022-03-09)
Postoperative cognitive dysfunction (POCD) is a common complication following anesthesia and surgery. General anesthetic isoflurane has potential neurotoxicity and induces cognitive impairments, but the exact mechanism remains unclear. Astrocytes form interconnected networks in the adult brain through gap junctions (GJs)
Yoshitaka Ohno et al.
Cells, tissues, organs, 194(5), 363-370 (2011-03-23)
Heat stress is one of the hypertrophic stimuli on mammalian skeletal muscle. Nuclear factor-κB (NF-κB) signaling plays an important role in the regulation of skeletal muscle mass. However, the effects of heat stress on NF-κB signaling in skeletal muscle cells
Igor Moura de Paiva et al.
Molecular pharmaceutics, 19(6), 1825-1838 (2022-03-11)
The disruption of polynucleotide kinase/phosphatase (PNKP) in colorectal cancer (CRC) cells deficient in phosphatase and tensin homolog (PTEN) is expected to lead to the loss of cell viability by a process known as synthetic lethality. In previous studies, we have
Cheng-Hsuan Ho et al.
Frontiers in physiology, 12, 725172-725172 (2021-12-21)
Water permeability of the kidney collecting ducts is regulated by the peptide hormone vasopressin. Between minutes and hours (short-term), vasopressin induces trafficking of the water channel protein aquaporin-2 to the apical plasma membrane of the collecting duct principal cells to
Clyde J Wright et al.
Reproductive sciences (Thousand Oaks, Calif.), 27(11), 2063-2074 (2020-06-17)
The transcription factor NFκB has been associated with the timing of menopause in a large human genome-wide association study. Furthermore, preclinical studies demonstrate that loss of Tumor necrosis factor alpha (Tnfα) or its receptor Tnfr2 slows primordial follicle growth activation
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.