5.09290

G 418 Sulfate Salt

OmniPur^® Grade, >=730 µg/mg

• Synonym(e): OmniPur^® G418 Sulfate, Cell Culture Tested, NMD Inhibitor I
• Empirische Formel (Hill-System): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• CAS-Nummer: 108321-42-2
• Molekulargewicht: 692.71
• MDL-Nummer: MFCD00058314
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 200
• Assay: ≥90% (HPLC)
• Form: powder
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• Kationenspuren: heavy metals: ≤100 ppm
• Lagertemp.: 10-30°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChIKey: UHEPSJJJMTWUCP-NKCAIAFTSA-N

Beschreibung

Allgemeine Beschreibung

Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neo^r/kan^r genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Biochem./physiol. Wirkung

Assay: ≥730 µg/mg

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Solubilization in H₂O at 100 mg/ml yields a clear, colorless solution.

Sonstige Hinweise

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

OMNIPUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H317 - H334
• P-Sätze: P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312
• Gefahreneinstufungen: Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3