5.09228

Y-27632

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem

• Synonym(e): InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested
• Empirische Formel (Hill-System): C₁₄H₂₁N₃O · 2HCl
• CAS-Nummer: 146986-50-7
• Molekulargewicht: 320.26
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• product name: Y-27632, InSolution, ≥95%, 10 mM aqueous solution, Sterile-Filtered, inhibitor of Rho-associated protein kinases
• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Lagertemp.: −20°C
• InChI: 1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11?,12?/m1/s1
• InChIKey: IYOZTVGMEWJPKR-VOMCLLRMSA-N

Beschreibung

Allgemeine Beschreibung

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.

Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.

Anwendung

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).

Biochem./physiol. Wirkung

Primary Target
ROCK

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Sonstige Hinweise

Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J.351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science.285, 895.
Uehata, M., et al. 1997. Nature.389, 990.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable