5.08838

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested

• Synonym(e): Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested, (2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
• Empirische Formel (Hill-System): C₂₂H₂₉N₇O₅ · 2HCl
• CAS-Nummer: 58-58-2
• Molekulargewicht: 544.43
• MDL-Nummer: MFCD00012691
• UNSPSC-Code: 12352101

Eigenschaften

• Assay: ≥98% (HPLC)
• Qualitätsniveau: 200
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Verunreinigungen: ≤10 EU/mg Endotoxin (dry weight)
• Lagertemp.: −20°C
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
• InChIKey: MKSVFGKWZLUTTO-USYAMCSGSA-N

Beschreibung

Allgemeine Beschreibung

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.

Biochem./physiol. Wirkung

Primary Target
RNA

Reversible: yes

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Supplied at 10 mg/ml in H₂O.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Sonstige Hinweise

Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Kaufman, S.H., et al. 1993. Cancer Res.53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol.216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res.216, 149.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°C): Not applicable