407256

IGF-II, CF, Mouse, Recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.75

Eigenschaften

• Assay: ≥97% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: <1.0 EU/μg protein Endotoxin
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, mouse insulin-like growth factor II (IGF-II) consisting of amino acids 25-91 and expressed in E. coli. Mouse IGF-II is synthesized as a 180 amino acid preproprecursor. Mature IGF-II is 91% and 97% amino acid identical to human and rat IGF-II, respectively.

Biochem./physiol. Wirkung

ED₅₀ = 2-10 ng/ml as measured in a serum-free cell proliferation assay using MCF-7 human breast adenocarcinoma cells.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA.

Rekonstituierung

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS.

Sonstige Hinweise

Ostrovsky, O., et al. 2009. Mol. Biol. Cell20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev.19, 111.
Belfiore, A., 2007. Curr. Pharm. Des.13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab.292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol.7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res.125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des.13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v.16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett.195,127.
Buchanan, C.M., et al. 2001. Biochem. J.360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci.57, 932.
Goya, L., et al. 1999. J. Biol. Chem.274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept.48, 77.
McCarthy, T.L., et al. 1992. Endocrinology130, 1303.
Zindy, F., et al. 1992. J. Hepatol.14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol.10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA85, 265.
Stempien, M.M., et al. 1986. DNA5, 357.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H319
• P-Sätze: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Eye Irrit. 2
• Lagerklassenschlüssel: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2