382129

HIV Reverse Transcriptase, Recombinant, E. coli

liquid, ≥5,000 units/mg protein, Nuclease-free

• Synonym(e): HIV RT
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 100
• Form: liquid
• Spezifische Aktivität: ≥10,000 units/mL; ≥5,000 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Methode(n): molecular cloning: suitable
• Fremdaktivität: Nuclease-free
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Anwendung

HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.

Physikalische Form

In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1