371958

H-8, Dihydrochloride

A potent, cell-permeable, reversible, and ATP-competitive inhibitor of cyclic-nucleotide-dependent protein kinases.

• Synonym(e): H-8, Dihydrochloride, N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor I
• Empirische Formel (Hill-System): C₁₂H₁₅N₃O₂S · 2HCl
• CAS-Nummer: 113276-94-1
• Molekulargewicht: 338.25
• MDL-Nummer: MFCD00036962
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 100
• Assay: >95% (TLC)
• Form: lyophilized
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Löslichkeit: water: 10 mg/mL
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• InChI: 1S/C12H15N3O2S.2ClH/c1-13-7-8-15-18(16,17)12-4-2-3-10-9-14-6-5-11(10)12;;/h2-6,9,13,15H,7-8H2,1H3;2*1H
• InChIKey: RJJLZYZEVNCZIW-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

A potent, cell-permeable, reversible, and ATP-competitive inhibitor of cyclic-nucleotide-dependent protein kinases. Inhibits protein kinase A (K_i = 1.2 µM), myosin light chain kinase (K_i = 68 µM), protein kinase C (K_i = 15 µM), and protein kinase G (K_i = 480 nM).

A potent, cell-permeable, reversible, and ATP-competitive, and highly active inhibitor of cyclic-nucleotide-dependent protein kinases. Inhibits myosin light chain kinase (K_i = 68 µM), protein kinase A (K_i = 1.2 µM), protein kinase C (K_i = 15 µM), and protein kinase G (K_i = 480 nM).

Biochem./physiol. Wirkung

Cell permeable: yes

Product competes with ATP.

Reversible: yes

Target K_i: 68 µM against MLCK

Warnhinweis

Toxicity: Standard Handling (A)

Angaben zur Herstellung

Further dilute with aqueous buffer just prior to use.

Sonstige Hinweise

Ido, M., et al. 1991. Br. J. Cancer 64, 1103.
Hagiwara, M., et al. 1987. Mol. Pharmacol. 31, 523.
Inagaki, M., et al. 1986. Mol. Pharmacol.29, 577.
Hidaka, H., et al. 1984. Biochemistry23, 5036.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable