16-204

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^®, biotin conjugate

clone 4G10®, Upstate^®, from mouse

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: biotin conjugate
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 4G10®, monoclonal
• Speziesreaktivität (Voraussage durch Homologie): all
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• Isotyp: IgG2bκ
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PID1(55022)

Beschreibung

Allgemeine Beschreibung

Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.

Spezifität

Tyrosine-phosphorylated proteins from all species.

Immunogen

Phosphotyramine-KLH

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Allgemeine Posttranslationsmodifikation

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10, biotin conjugate validated for use in WB.

Qualität

Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Zielbeschreibung

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Physikalische Form

Protein G Purified

Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.

Lagerung und Haltbarkeit

Stable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2