1.50465

SeQuant^™ ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 150 mm x 0.075 mm capillay Column

• UNSPSC-Code: 41115709

Eigenschaften

• product name: SeQuant^™ ZIC-HILIC^™ (5µm,200Å) 150 x 0.075 mm, suitable for HPLC
• Materialien: stainless steel column
• Qualitätsniveau: 100
• Agentur: suitable for USP L114
• Produktlinie: SeQuant^™
• Leistungsmerkmale: endcapped: no
• Parameter: 400 bar pressure; 70 °C max. temp.
• Methode(n): HPLC: suitable; LC/MS: suitable (Capillary LC); mass spectrometry (MS): suitable (High)
• Säule L × ID: 15 cm × 0.075 mm
• Matrix: fully porous particle
• Aktive Matrixgruppe: ZIC-HILIC bonding phase
• Partikelgröße: 5 μm
• Porengröße: 200 Å pore size
• Betriebs-pH-Wert: 2-7.5
• Trenntechnik: hydrophilic interaction (HILIC)
• Lagertemp.: no temp limit

Beschreibung

Allgemeine Beschreibung

SeQuant^™ ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^™ ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Anwendung

• 2-Homoectoine: An Additional Member of the Ectoine Family from Phyto- and Bacterioplankton Involved in Osmoadaptation.: This study explores the discovery of 2-homoectoine, a new member of the ectoine family, and its role in osmoadaptation in phyto- and bacterioplankton, highlighting its potential in biotechnological applications (Azizah and Pohnert, 2024).



• An efficient strategy with a synergistic effect of hydrophilic and electrostatic interactions for simultaneous enrichment of N- and O-glycopeptides.: The research presents a novel method for enriching glycopeptides using synergistic hydrophilic and electrostatic interactions, showcasing the efficiency of ZIC-HILIC columns in proteomics (Hu et al., 2024).



• Improved analysis ZIC-HILIC-HCD-Orbitrap method for mapping the glycopeptide by mass spectrometry.: This paper describes enhancements in glycopeptide mapping using a ZIC-HILIC-HCD-Orbitrap method, emphasizing its utility in detailed proteomic analyses (Li et al., 2023).



• Isomeric separation of native N-glycans using nano zwitterionic-hydrophilic interaction liquid chromatography column.: The study highlights the separation of isomeric N-glycans using nano-ZIC-HILIC columns, demonstrating advancements in glycomic profiling techniques (Daramola et al., 2023).

Rechtliche Hinweise

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany