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Key Documents

14-173-M

Sigma-Aldrich

MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.

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About This Item

UNSPSC-Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Rekombinant

expressed in E. coli

Mol-Gew.

Mw 67.8 kDa

Hersteller/Markenname

Upstate®

Methode(n)

activity assay: suitable (kinase)

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Allgemeine Beschreibung

N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2

Biochem./physiol. Wirkung

Protein Target: MAPK2
Target Sub-Family: CMGC

Verpackung

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.

Qualität

Routinely evaluated by phosphorylation myelin basic protein

Physikalische Form

Glutathione agarose; Ni2+/NTA agarose
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Lagerung und Haltbarkeit

Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.

Sonstige Hinweise

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
Sutherland, C, et al.
European Journal of Biochemistry, 212, 581-588 (1993)
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Sanghera, J S, et al.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
B Zhang et al.
The Journal of biological chemistry, 271(50), 31771-31774 (1996-12-13)
Peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation and the regulation of adipocyte gene expression. Insulin also serves to promote adipogenesis. We report that insulin and a PPARgamma ligand (thiazolidinedione (TZD)) stimulate in a synergistic manner
alpha7 Nicotinic Acetylcholine Receptors Mediate {beta}-Amyloid Peptide-induced Tau Protein Phosphorylation
Wang, H. Y., et al
The Journal of Biological Chemistry, 278, 31547-31553 (2003)
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
Sanghera, J S, et al.
The Journal of Biological Chemistry, 265, 52-57 (1990)

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