09-482

Anti-PI3 Kinase Antibody, p110β

from rabbit, purified by affinity chromatography

• Synonym(e): PI3-kinase p110 subunit alpha, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform, ph
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human, canine
• Speziesreaktivität (Voraussage durch Homologie): rat (immunogen homology), mouse (immunogen homology), dog (immunogen homology), monkey (immunogen homology)
• Methode(n): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_006210.1
• UniProt-Hinterlegungsnummer: P42336
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PIK3CB(5291)

Beschreibung

Allgemeine Beschreibung

PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

Spezifität

Recognizes the internal domain of PI3 Kinase, p110β.

Immunogen

Epitope: Internal Domain

KLH-conjugated synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110β.

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
PI3K-, Akt-, & mTOR-Signalübertragung

Anti-PI3 Kinase Antibody, p110β is an antibody against PI3 Kinase for use in WB, IP, IC, IH, ELISA.

ELISA:
This antibody was tested and determined to work for ELISA at 0.5 ng/well.

Qualität

Evaluated by Western Blot on HEK-293 cell lysate.
Western Blot Analysis: 0.05 - 0.1 μg/mL of this antibody detected endogenous PI3 Kinase, p110β in lysate from HEK-293 cells.

Zielbeschreibung

110 kDa

Verlinkung

Replaces: 04-399

Physikalische Form

Affinity purified

Antigen affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl, with 0.05% NaN3.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Hinweis zur Analyse

Control
HEK-293 cell lysate.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable