09-433

Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody

Upstate^®, from rabbit

• Synonym(e): HIRS-1, Insulin Receptor Substrate 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Upstate^®
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NM_005544
• UniProt-Hinterlegungsnummer: P35568
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (pTyr628/pTyr632 )
• Angaben zum Gen: human ... IRS1(3667)

Beschreibung

Allgemeine Beschreibung

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Spezifität

Predicted to cross react with mouse and rat based on 100% sequence homology.

Recognizes phospho-IRS1 (Tyr628) mouse/ (Tyr632) human

Immunogen

peptide corresponding to amino acids encompassing and including phosphorylated Tyr628 in mouse and Tyr632 in human of IRS1

Anwendung

Research Category
Zelluläre Signaltransduktion

Research Sub Category
Insulin-/stoffwechselassoziierte Signalübertragung

Anti-phospho-IRS1 (Tyr628) mouse/ (Tyr632) human Antibody is an antibody against phospho-IRS1 (Tyr628) mouse/ (Tyr632) human for use in WB.

Qualität

routinely evaluated by western blot on lysates from untreated and insulin-treated (30 minutes) HEK 293 cells

Zielbeschreibung

170 kDa

Physikalische Form

Affinity purified

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.

Format: Purified

Lagerung und Haltbarkeit

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2