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07-1496

Sigma-Aldrich

Anti-CaM Kinase II Antibody

from rabbit, purified by affinity chromatography

Synonym(e):

Anti-CAMKA, Anti-CaMKIINalpha, Anti-CaMKIIalpha, Anti-MRD53, Anti-MRT63

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

mouse, chicken, bovine, hamster, human, rabbit, rat, guinea pig

Methode(n)

western blot: suitable

Isotyp

IgG

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CAMK2A(815)

Allgemeine Beschreibung

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Spezifität

Recognizes proteins of 50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II α subunits expressed in sf9 insect cells are also detected. As the sequences of the rat α, δ, and γ isoforms are conserved over this amino terminus region, this antibody is expected to recognize the δ, γ, and α isoforms. Antibody is not expected to cross-react with the β isoform. Proteins of unknown identity may be detected on immunoblot analysis with some lysates (40, 45 and 90 kDa).

Immunogen

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of Rat CaMK II

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
GPCR-, cAMP/cGMP- & kalziumabhängige Signalübertragung
Anti-CaM Kinase II Antibody is an antibody against CaM Kinase II for use in WB.
Optimal working dilutions must be determined by end user.

Qualität

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.

Zielbeschreibung

50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms.

Verlinkung

Replaces: AB3111

Physikalische Form

Affinity purified
Purified rabbit polyclonal IgG in buffer containing borate buffered saline and phosphate buffered saline containing 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. For optimal storage, aliquot and store at -20°C or -70°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
HEK293 cell lysate

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

C Rongo et al.
Nature, 402(6758), 195-199 (2000-01-26)
Synaptic connections undergo a dynamic process of stabilization or elimination during development, and this process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. The type II calcium- and calmodulin-dependent protein kinase
R M Tombes et al.
Biochimica et biophysica acta, 1452(1), 1-11 (1999-10-19)
Over two dozen alternative splice variants of CaMK-II, the type II Ca(2+)/CaM-dependent protein kinase, are encoded from four genes (alpha, beta, gamma and delta) in mammalian cells. Isozymes of alpha and beta CaMK-II are well characterized in brain; however, an
F Gardoni et al.
FEBS letters, 456(3), 394-398 (1999-08-26)
Ca2+/calmodulin-dependent protein kinase II (CaMKII), a multifunctional, widely distributed enzyme, is enriched in post-synaptic densities (PSDs). Here, we demonstrate that CaMKII binds to a discrete C-terminal region of the NR2A subunit of NMDA receptors and promotes the phosphorylation of a
Y H Koh et al.
Cell, 98(3), 353-363 (1999-08-24)
Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated
Anand M Prasad et al.
Vascular pharmacology, 87, 172-179 (2016-09-24)
Angiotensin-II (Ang-II) is a well-established mediator of vascular remodeling. The multifunctional calcium-calmodulin-dependent kinase II (CaMKII) is activated by Ang-II and regulates Erk1/2 and Akt-dependent signaling in cultured smooth muscle cells in vitro. Its role in Ang-II-dependent vascular remodeling in vivo

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