07-1285

Anti-acetylated-Src (Lys430) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): SRC
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Methode(n): cell based assay: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005408
• UniProt-Hinterlegungsnummer: P12931
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: acetylation (Lys430)
• Angaben zum Gen: human ... SRC(6714)

Beschreibung

Allgemeine Beschreibung

Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.

Spezifität

This antibody recognizes Src acetylated at Lys430.

Immunogen

Epitope: Acetylated Lys430

KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelett

This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Function Assay for the detection of acetylated-Src (Lys430).

Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.

Qualität

Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.

Zielbeschreibung

~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HEK293T cells transfected with myc-tagged Src and CBP

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids