06-1064

Anti-ITK/EMT/TSK Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): IL2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_005537
• UniProt-Hinterlegungsnummer: Q08881
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ITK(3702)

Beschreibung

Allgemeine Beschreibung

Tyrosine-protein kinase ITK/TSK (UniProt: Q08881; also known as EC:2.7.10.2, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LYK) gene (Gene ID: 3702) in human. ITK/TSK is a protein tyrosine kinase that plays an essential role in regulation of the adaptive immune response. It regulates the development, function, and differentiation of conventional T-cells and non-conventional natural killer T-cells. It contains four zinc binding sites. It s N-terminal PH domain allows it to be recruited to the plasma membrane by an activated PI3 kinase. This domain also contains a proline-rich region (PRR). The adjoining SH3 domain binds to PRR. The SH2 domain is required for binding tyrosine-phosphorylated substrates and the kinase domain located in the C-terminal region is required for tyrosine phosphorylation. ITK/TSK is phosphorylated at Tyr512 in the activation loop of the kinase domain by LCK and subsequent autophosphorylation at Tyr180, which lies within the substrate-binding sequence in the SH3 domain, allows the kinase activation. Mutations in ITK gene are reported to cause lymphoproliferative syndrome 1, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus that can lead to fatal outcome.

Spezifität

Predicted to cross-react with mouse based on 100% sequence homology.

Recognizes Itk/Emt/Tsk, Mr ~72 kDa.

Immunogen

GST-tagged recombinant fragment cvorresponding to the first 270 amino acids from the N-terminal half of human Tyrosine-protein kinase ITK/TSK spanning the PH, SH3 and a small portion of the SH2 domains.

Anwendung

Research Category
Zelluläre Signaltransduktion

Entzündung & Immunologie

Research Sub Category
Immunologische Signalübertragung

Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated ITK/EMT/TSK in 500 µg of Jurkat cell lysate.

This rabbit polyclonal Anti-ITK/EMT/TSK, Cat. No. 06-1064 is a rabbit polyclonal antibody that targets protein tyrosine kinase ITK/TSK and is tested for use in Immunoprecipitation and Western blotting.

Qualität

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ITK/EMT/TSK in 10 µg of Jurkat cell lysate.

Zielbeschreibung

~71 kDa observed; 71.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Verlinkung

Replaces: 06-546

Physikalische Form

Affinity purified

Purified rabbit polycloncal in buffer containing PBS with 0.05% sodium azide and 50% glycerol.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Hinweis zur Analyse

Control
Jurkat cell lysates

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2