05-988

Anti-Pras40 Antibody, Clone 73P21

clone 73P21, Upstate^®, from mouse

• Synonym(e): 40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 73P21, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Upstate^®
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NP_001092102
• UniProt-Hinterlegungsnummer: Q96B36
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... AKT1S1(84335)

Beschreibung

Allgemeine Beschreibung

PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

Spezifität

Recognizes PRAS40.

This antibody does not cross react with mouse.

Immunogen

Recombinant human PRAS40 (full-length) expressed in E. coli.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
PI3K-, Akt-, & mTOR-Signalübertragung

Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.

Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Qualität

Routinely evaluated by western blot.

Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Zielbeschreibung

40 kDa

Physikalische Form

Affinity purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 years at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
RIPA lysates of HeLa cells.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable