Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

05-1324

Sigma-Aldrich

Anti-phospho-Histone H1 Antibody, clone 12D11

culture supernatant, clone 12D11, from mouse

Synonym(e):

H1 histone family, member 5, Histone H1a, histone 1, H1b, histone cluster 1, H1b

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

culture supernatant

Antikörper-Produkttyp

primary antibodies

Klon

12D11, monoclonal

Speziesreaktivität

human, mouse, bovine, vertebrates

Methode(n)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgMκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (not specified)

Angaben zum Gen

bovine ... H1-5(527304)
human ... H1-5(3009)

Allgemeine Beschreibung

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Spezifität

Broad species cross-reactivity is expected, based on sequence homology.
Recognizes phosphorylated Histone H1, Mr 32 kDa.

Immunogen

The immunogen was KLH-conjugated bovine nuclear extract.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
Histone
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect CDK (cyclin dependent kinase) phosphorylated Histone H1 in benign tissue sections.1

Immunocytochemistry:
This antibody has been demonstrated by an outside laboratory to show positive immunostaining for phosphorylated histone H1 in HUSK (human fetal primary myoblasts) cells fixed with 3.7% paraformaldehyde followed by permeabilization with 0.2% Triton X-100.2

Immunoprecipitation:
This antibody has been demonstrated by an outside laboratory to immunoprecipitate phosphorylated histone H1 using solid phase beads bound to anti-mouse IgM.
Use Anti-phospho-Histone H1 Antibody, clone 12D11 (Mouse Monoclonal Antibody) validated in IHC, ICC, IP, WB to detect phospho-Histone H1 also known as Histone H1a, histone 1 H1b.

Qualität

Western Blot Analysis:
1:1000 dilution of this antibody detected Histone H1 in colcemid-treated HeLa lysate.

Zielbeschreibung

~32 kDa

Physikalische Form

Supplied as 200 μL of culture supernatant with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
HeLa cell lysate, treated with colcemid.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Growth factors that repress myoblast differentiation sustain phosphorylation of a specific site on histone H1.
Cole, F, et al.
The Journal of Biological Chemistry, 268, 1580-1585 (1993)
Lisa L Hall et al.
The Journal of cell biology, 186(4), 491-507 (2009-08-26)
How XIST RNA strictly localizes across the inactive X chromosome is unknown; however, prophase release of human XIST RNA provides a clue. Tests of inhibitors that mimic mitotic chromatin modifications implicated an indirect role of PP1 (protein phosphatase 1), potentially
Lasonolide A, a potent and reversible inducer of chromosome condensation.
Zhang, YW; Ghosh, AK; Pommier, Y
Cell Cycle null
Qing-Zhang Tuo et al.
Cell death & disease, 9(9), 870-870 (2018-08-31)
CDK5 activation promotes ischemic neuronal death in stroke, with the recognized activation mechanism being calpain-dependent p35 cleavage to p25. Here we reported that CDK5-Tyr15 phosphorylation by zinc induced CDK5 activation in brain ischemic injury. CDK5 activation and CDK5-Tyr15 phosphorylation were

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.