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100500P

Avanti

E. coli Extract Total

Avanti Research - A Croda Brand

Synonym(e):

E. coli Total Lipid Extract

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352211
NACRES:
NA.25

Form

powder

Verpackung

pkg of 1 × 1 g (100500P-1g)
pkg of 1 × 100 mg (100500P-100mg)
pkg of 5 × 100 mg (100500P-500mg)

Hersteller/Markenname

Avanti Research - A Croda Brand

Lipid-Typ

lipid extracts

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Anwendung

E. coli Extract Total has been used in the preparation of unilamellar vesicles in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins and in the reconstitution of proteoliposomes.

Biochem./physiol. Wirkung

The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.

Verpackung

5 mL Clear Glass Sealed Ampule (100500P-100mg)
5 mL Clear Glass Sealed Ampule (100500P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100500P-1g)

Rechtliche Hinweise

Avanti Research is a trademark of Avanti Polar Lipids, LLC

auch häufig zusammen mit diesem Produkt gekauft

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Analysenzertifikate (COA)

Lot/Batch Number

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Membrane insertion of small proteins
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Sophie J Hesketh et al.
Biochimica et biophysica acta. Biomembranes, 1862(5), 183192-183192 (2020-01-17)
Membrane proteins are traditionally extracted and purified in detergent for biochemical and structural characterisation. This process is often costly and laborious, and the stripping away of potentially stabilising lipids from the membrane protein of interest can have detrimental effects on
Lipid compositions in Escherichia coli and Bacillus subtilis during growth as determined by MALDI-TOF and TOF/TOF mass spectrometry
Gidden J, et al.
International Journal of Mass Spectrometry, 283(1-3), 178-184 (2009)

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