ZRB2629

Anti-MLC-2 Antibody, clone 1B16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cardiac myosin light chain 2, MLC-2s/v, MLC-2v, Myosin light chain 2, slow skeletal/ventricular muscle isoform, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, Ventricular myosin light chain 2
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B16, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 18.79 kDa; observed mol wt ~19 kDa
• purified by: using Protein A
• species reactivity: human, mouse, rat, bovine
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_000423.2
• UniProt accession no.: P10916
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... MYL2(4633)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B16 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects MLC-2. It targets an epitope within 20 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Myosin regulatory light chain 2 (MLC2).

Application

Quality Control Testing

Evaluated by Western Blotting in fetal Bovine heart endothelial (FBHE) cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MLC-2 in fetal Bovine heart endothelial (FBHE) cell lysate.

Tested Applications

Immunocytochemistry Analysis (ICC): A 1:100 dilution of this antibody detected MLC-2 in C2C12 cells.

Affinity Binding Assay:: A representative lot of this antibody bound MLC-2 peptide with a KD of 1.5 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:1,000 Dilution of this antibody detected MLC-2 in human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (UniProt: P10916; also known as MLC-2, ; MLC-2v, Cardiac myosin light chain 2, Myosin light chain 2, slow skeletal/ventricular muscle isoform, MLC-2s/v, Ventricular myosin light chain 2) is encoded by the MYL2 (also known as MLC2) gene (Gene ID: 4633) in human. Myosins belong to a family of actin-based motor molecular motors with ATPase activity that is essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Fast and slow mammalian muscle myosins differ in their heavy chain sequences. Muscles expressing the two isoforms contract at markedly different velocities. MLC-2 is highly expressed in type I muscle fibers and plays a role in heart development and function. It is phosphorylated by myosin light chain kinase 3 (MYLK3) and myosin light chain kinase 2 (MYLK2), which promotes cardiac muscle contraction and function. Following phosphorylation, it plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion, as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events are reported to slow down myosin kinetics and prolong duty cycle resulting that results in cooperative recruitment of myosin to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament Ca2+ sensitivity to force. During cardiogenesis, it plays an early role in cardiac contractility by promoting cardiac myofibril assembly. Mutations in MYL2 gene are known to cause familial hypertrophic cardiomyopathy that is characterized by ventricular hypertrophy, dyspnea, syncope, palpitations, and chest pain. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Manivannan, SN., et al. (2020). PLoS Genet. 16(5); e1008639; Sheikh, F., et al. (2015). Gene. 569(1); 14-20; Iorga, B., et al. (2007). J. Biol. Chem. 282(6); 3559-3570).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable