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Key Documents

SRP2018

Sigma-Aldrich

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

E1A

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

biological source

Saccharomyces cerevisiae
canine

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~33 kDa

packaging

pkg of 4 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

100 μg/mL

technique(s)

electrophoretic mobility shift assay: suitable

color

colorless to clear

NCBI accession no.

J04368
NM_001184062

UniProt accession no.

P04386

shipped in

dry ice

storage temp.

−70°C

Gene Information

Saccharomyces cerevisiae ... GAL4(855828)

Biochem/physiol Actions

The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
A J Berk et al.
Cell, 17(4), 935-944 (1979-08-01)
L Keegan et al.
Science (New York, N.Y.), 231(4739), 699-704 (1986-02-14)
The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription.
T Kodadek
Cellular & molecular biology research, 39(4), 355-360 (1993-01-01)
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5'-CGGN5(T/A)N5CCG-3'. We review the general requirements that must be met for

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