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About This Item
Empirical Formula (Hill Notation):
C38H50N3O6Cl
CAS Number:
Molecular Weight:
680.27
UNSPSC Code:
12352107
NACRES:
NA.77
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Quality Level
Assay
≥98% (HPLC)
form
powder
color
blue to black
solubility
DMSO: 2 mg/mL, clear
storage temp.
2-8°C
General description
GlucoseCy5 is a cell-permeable, Cy5-linked 1-amino-1-deoxy-β-glucose fluorescent tracer. It is used for glucose transporter (GLUT)-mediated molecular sensing and bioimaging. GlucoseCy5 demonstrates superior specificity, sensitivity, and non-toxicity over 2-NBDG. λ emission & excitation ~ 644 nm & 665 nm. Suggested optimum concentration and incubation for cellular uptake: 50 μM & 20 min.
Application
GlucoseCy5 has been used as a component in serum-free Dulbecco′s modified eagle medium (DMEM) for fluorescence-activated cell sorting (FACS) and glucose-uptake assay.
Biochem/physiol Actions
fluorescent tracer for glucose transporter (GLUT)-mediated molecular sensing and bioimaging
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Hu Xu et al.
Biochemical and biophysical research communications, 474(2), 240-246 (2016-04-02)
Two novel cyanine-based 1-amino-1-deoxy-β-glucose conjugates (Glu-1N-Cy3 and Glu-1N-Cy5) were designed, synthesized and their fluorescence characteristics were studied. Both Glu-1N-Cy3 and Glu-1N-Cy5 accumulate in living HT29 human colon cancer cells, which overexpress glucose transporters (GLUTs). The cellular uptake of the bioprobes
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