SCJ4600055

CF^™ 680 succinimidyl ester

suitable for fluorescence, ≥90% (TLC)

• UNSPSC Code: 12352203

Properties

• Quality Level: 100
• Assay: ≥90% (TLC)
• form: solid
• mol wt: ~3,241 g/mol (free acid)
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• ε (extinction coefficient): 210000 at 681 nm
• absorbance ratio: A_{280/681 nm} 0.09
• fluorescence: λ_ex 680 or 685 nm (Flow cytometry laser line); λ_ex 680 or 685 nm (Microscopy laser line); λ_ex 681 nm; λ_em 698 nm
• suitability: suitable for fluorescence
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

CF^™680 and CF^™680R are two outstanding near-IR dyes excitable at about 680 nm with emission at about 700 nm. The two dyes each have unique properties suitable for different application needs. CF^™680 is a highly water-soluble cyanine-based dye with a molecular weight of ~3000. This dye is excellent for labeling antibodies, producing the brightest fluorescence and highest signal-to-noise ratio in immunostaining among spectrally similar dyes, such as Cy^®5.5, Alexa Fluor^™ 680, DyLight^® 680 and IRDye^® 680. However, because of its relatively large molecular size, CF^™680 is not recommended for labeling nucleic acids or relatively small biomolecules, for which our CF^™680R is better suited.

Features and Benefits

1. The brightest among spectrally similar 680 nm dyes. 2. Superior signal-to-noise ratio in immunostaining. 3. Highly water-soluble and pH-insensitive.

Preparation Note

Protect from light and moisture

Reconstitution

Dissolve in DMSO

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Cy is a registered trademark of Cytiva

DyLight is a registered trademark of Pierce Biotechnology, Inc.

IRDye is a registered trademark of LI-COR, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable