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Key Documents

SAB2702291

Sigma-Aldrich

Monoclonal Anti-mCherry antibody produced in mouse

clone GT857, affinity isolated antibody

Synonym(s):

Anti Mcherry Antibody, Anti mCherry Antibody - Monoclonal Anti-mCherry antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT857, monoclonal

form

buffered aqueous solution

concentration

1mg/mL

technique(s)

immunohistochemistry: suitable
western blot: 1:500-1:3,000

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The acceptor (mCherry) is a monomeric protein obtained from stony-coral DsRed. It is expressed in the cytoplasm.

Immunogen

Full length mCherry recombinant protein

Application

Suggested starting dilutions are as follows: IHC: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

The acceptor (mCherry) possess high photostability and shows resistance to photobleaching. It acts as a good FRET (fluorescence resonance energy transfer) acceptor. mCherry helps in real-time analysis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lingxin Zhang et al.
Drug metabolism and disposition: the biological fate of chemicals, 49(5), 395-404 (2021-03-05)
SLCO1B1 (solute carrier organic anion transporter family member 1B1) is an important transmembrane hepatic uptake transporter. Genetic variants in the SLCO1B1 gene have been associated with altered protein folding, resulting in protein degradation and decreased transporter activity. Next-generation sequencing (NGS)
In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.
Vacchina P and Morales MA
Antimicrobial Agents and Chemotherapy, 58(3), 1825-1828 (2014)
Quantitative FRET Analysis With the E0GFP?mCherry Fluorescent Protein Pair.
Albertazzi L, et al.
Photochemistry and Photobiology, 85(1), 287-297 (2009)
Yasuomi Urano et al.
Cells, 9(2) (2020-02-08)
Amyloid-β (Aβ) peptides play a crucial role in the pathogenesis of Alzheimer's disease (AD). Aβ production, aggregation, and clearance are thought to be important therapeutic targets for AD. Curcumin has been known to have an anti-amyloidogenic effect on AD. In
Yuming Cao et al.
International journal of molecular sciences, 20(6) (2019-03-27)
The catalytically inactive mitogen-activated protein (MAP) kinase phosphatase, MK-STYX (MAPK (mitogen-activated protein kinase) phosphoserine/threonine/tyrosine-binding protein) interacts with the stress granule nucleator G3BP-1 (Ras-GAP (GTPase-activating protein) SH3 (Src homology 3) domain-binding protein-1), and decreases stress granule (stalled mRNA) formation. Histone deacetylase

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