PCG3003
TruPAGE™ Tris-MOPS SDS Express Running Buffer
20 ×
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About This Item
UNSPSC Code:
12352201
NACRES:
NB.22
Recommended Products
concentration
20 ×
compatibility
for use with Sigma-Aldrich TruPAGE Precast Gels
storage temp.
room temp
Application
TruPAGE™ Tris-MOPS SDS Express Running Buffer has been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Features and Benefits
TruPAGE Tris-MOPS SDS Express Running Buffer is specially formulated to be used with TruPAGE Precast Gels. This running buffer will provide increased resolution for small MW proteins, and will noticeably shorten the run time. This running buffer needs to be supplemented with a 1X working concentration of TruPAGE Antioxidant (PCG3007) to prevent protein reoxidation during electrophoresis when running reduced protein samples. Please refer to the gel migration chart to select the appropriate precast gel and running buffer combination for your experimental needs.
Legal Information
TruPAGE is a trademark of Sigma-Aldrich Co. LLC
configured for
Product No.
Description
Pricing
related product
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Detection of phosphorylated Akt and MAPK in cell culture assays.
Molgaard S, et al.
MethodsX, 3, 386-398 (2016)
Simon Molgaard et al.
MethodsX, 3, 386-398 (2016-06-09)
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite
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