P8038
Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonym(s):
Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
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About This Item
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Specificity
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Application
The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Biochem/physiol Actions
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Physical properties
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Heena Kumra et al.
PLoS biology, 16(7), e2004812-e2004812 (2018-07-24)
Fibronectin (FN) exists in two forms-plasma FN (pFN) and cellular FN (cFN). Although the role of FN in embryonic blood vessel development is well established, its function and the contribution of individual isoforms in early postnatal vascular development are poorly
Sharon L Barrans et al.
British journal of haematology, 117(2), 322-332 (2002-04-26)
Diffuse large B-cell lymphomas (DLBCL) are a heterogeneous group of tumours, varying in clinical features, immunophenotype and cytogenetics. The aim of this study was to investigate the prognostic significance of BCL6 gene rearrangement at the 3q27 locus in patients with
Joseph W Starnes et al.
Journal of applied physiology (Bethesda, Md. : 1985), 102(5), 1793-1798 (2007-02-17)
Exercise provides cardioprotection against ischemia-reperfusion injury, a process involving mitochondrial reactive oxygen species (ROS) generation and calcium overload. This study tested the hypotheses that isolated mitochondria from hearts of endurance-trained rats have decreased ROS production and improved tolerance against Ca(2+)-induced
N V Naoumov et al.
Journal of clinical pathology, 41(7), 793-798 (1988-07-01)
A rapid and reproducible technique for in situ hybridisation, using biotin labelled probes for the Y chromosome, human DNA, hepatitis B virus DNA and cytomegalovirus DNA on formalin fixed, paraffin embedded liver tissue, was developed. The degree of proteolytic digestion
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal
Protocols
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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