P5493

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

• Synonym(s): PBS
• MDL number: MFCD00131855
• UNSPSC Code: 12161700
• eCl@ss: 32129211

Properties

• product name: Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture
• grade: BioPerformance Certified; for molecular biology
• Quality Level: 100; 200
• sterility: 0.2 μm filtered
• form: solution
• storage condition: (Tightly closed)
• technique(s): cell culture | mammalian: suitable; immunocytochemistry: suitable; immunofluorescence: suitable
• impurities: Bioburden, tested; endotoxin, tested; ≤5 ppm heavy metals (as Pb)
• color: colorless
• pH: 7.2-7.6 (25 °C)
• solubility: water: soluble
• cation traces: heavy metals (as Pb): ≤5 ppm
• suitability: suitable for ; suitable for flow cytometry; suitable for microscopy
• application(s): advanced drug delivery
• foreign activity: DNAse, none detected; NICKase, none detected; Protease, none detected; RNAse, none detected
• SMILES string: OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]
• InChI: InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5
• InChI key: LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Description

Application

Phosphate buffered saline has been used:

• as a cell wash solution in human dental pulp stem cells (DPSCs)
• for perfusion studies in dissected brain samples
• as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
• as wash solution for ovary and sperm during in vitro culture and fertilization

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.

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Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable