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Key Documents

M0660

Sigma-Aldrich

Mycoplasma Agar

powder, suitable for microbiology

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.74

form

powder

solubility

H2O: 35.5 g/L

application(s)

microbiology

General description

Boil to dissolve the agar and adjust pH to 7.8±0.2. Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.
The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Application

Recommended for the cultivation of mycoplasma

Components

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement, 10.0 agar.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.

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