LYSISO1

Lysosome Isolation Kit

sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells

• EC Number: 233-140-8
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• Quality Level: 200
• usage: sufficient for 20 mL (packed cells); sufficient for 25 g (tissue)
• technique(s): centrifugation: suitable; fractionation: suitable
• storage temp.: 2-8°C

Description

General description

Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.

Application

Lysosome Isolation Kit has been used:

• in the isolation of cytosol and lysosome
• to obtain enriched light and heavy lysosomal fractions from cultured cells
• in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
• in lysosome purification

The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.

Features and Benefits

• Generates functional organelles for metabolism and disease research
• Includes protease inhibitor to manage degradation
• Measures integrity with included Neutral Red dye
• Choose from multiple options to obtain enriched fractions
• Enriched fractions for intact and functional lysosomes from tissues and cells
• Suitable for functional studies including protein degradation

Kit Components Also Available Separately

• P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C