GE28-9537-67
HiTrap® TALON® Crude
Cytiva 28-9537-67, pack of 5 × 5 mL
Synonym(s):
Crude TALON Column, HiTrap TALON, TALON Crude Column
About This Item
Recommended Products
ligand
TALON® Superflow Cobalt-IMAC
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-9537-67
parameter
1 ml/min - 4 ml/min flow rate
3 bar
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≥20 mg binding capacity (histidine-tagged protein)
storage temp.
2-8°C
General description
Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
Features and Benefits
- Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
- Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
- HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
Storage and Stability
Analysis Note
Other Notes
Legal Information
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
Protocols
This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.
This page how to isolate proteins from inclusion bodies using GE Healthcare products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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