GE28-4009-90
His MultiTrap™ Fast Flow
Cytiva 28-4009-90, pack of 4 plates
Synonym(s):
His Tag Affinity Purification
About This Item
Recommended Products
material
polypropylene and polyethylene
packaging
pack of 4 plates
manufacturer/tradename
Cytiva 28-4009-90
size
127.8 mm × 85.5 mm × 30.6 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
suitability
suitable for bioprocess medium
General description
Application
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
Features and Benefits
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from GE Healthcare.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Protocols
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from GE Healthcare.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
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