F3022
Anti-β-Actin–FITC antibody, Mouse monoclonal
clone AC-15, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-β-Actin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-15, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
should not react with
Dictyostelium discoideum
technique(s)
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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General description
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.
Immunogen
slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Application
Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- direct immunofluorescent staining
- dual immuno-fluorescent staining
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Biochem/physiol Actions
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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