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Key Documents

F0679

SAFC

Fetal Bovine Serum

non-USA origin, γ-irradiated, sterile-filtered, suitable for cell culture

Pharma Manufacturing

Synonym(s):

FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.75
biological source:
bovine fetus
origin:
non-USA origin
sterility:
γ-irradiated
sterile-filtered
shipped in:
dry ice

biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated
sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

available only in EU
not available in Canada

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
FBS is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Analysis Note

  • Endotoxin and hemoglobin tested

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

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Fetal Bovine Serum USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Sigma-Aldrich

F4135

Fetal Bovine Serum

Beatriz Vera-Pérez et al.
Journal of histotechnology, 42(4), 183-192 (2019-09-04)
Human adipose stem cells can differentiate into various mesodermic lineages, including adipogenic, osteogenic, chondrogenic, myogenic and endothelial pathways. In addition, these cells types possess immunomodulatory properties, potentially useful for autoimmune and autoinflammatory diseases. However, single-cell expanded clones have shown that

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