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D6293

Sigma-Aldrich

pUC18 DNA Hae III Digest

for DNA electrophoresis

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

form

liquid

usage

200 uses (0.1 mg size)
40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

Components

Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Preparation Note

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen
Mrazek J and Kopecny J
Folia Microbiologica, 46(1), 63-65 (2001)

Articles

Markers and Ladders for Gel Electrophoresis

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

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