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D0942

Anti-DHFR, C-terminal antibody produced in rabbit

Name: Anti-DHFR, C-terminal antibody produced in rabbit
Brand: SIGMA

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Dihydrofolate reductase

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About This Item

MDL number:

MFCD05665211

UNSPSC Code:

12352203

NACRES:

NA.56

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

concentration

1.0-1.5 mg/mL

technique(s)

immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR
western blot (chemiluminescent): 0.5-1.0 μg/mL using 50-100 ng of purified recombinant DHFR, or 293T human embryonic kidney cell extract, or Jurkat human acute T Cell leukemia cell extract

UniProt accession no.

P00374

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Specificity

DHFR and DHFR fusion proteins. The epitope resides within amino acids 171-185 of mouse DHFR.

Immunogen

amino acids 171-185 of mouse DHFR, conjugated to KLH via an N-terminal added cysteine residue.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Purification and kinetic properties of human recombinant dihydrofolate reductase produced in Bombyx mori chrysalides.

Soledad Chazarra et al.

Applied biochemistry and biotechnology, 162(7), 1834-1846 (2010-04-16)

Recent reports describe the inhibition of human dihydrofolate reductase (hDHFR) by natural tea polyphenols. This finding could explain the epidemiologic data on their prophylactic effects for certain forms of cancer, and it raises the possibility that natural and synthetic polyphenols

Disease-causing mutations in subunits of OXPHOS complex I affect certain physical interactions.

Gilad Barshad et al.

Scientific reports, 9(1), 9987-9987 (2019-07-12)

Mitochondrial complex I (CI) is the largest multi-subunit oxidative phosphorylation (OXPHOS) protein complex. Recent availability of a high-resolution human CI structure, and from two non-human mammals, enabled predicting the impact of mutations on interactions involving each of the 44 CI

Protein-fragment complementation assays for large-scale analysis, functional dissection and dynamic studies of protein-protein interactions in living cells.

Stephen W Michnick et al.

Methods in molecular biology (Clifton, N.J.), 756, 395-425 (2011-08-27)

Protein-fragment Complementation Assays (PCAs) are a family of assays for detecting protein-protein interactions (PPIs) that have been developed to provide simple and direct ways to study PPIs in any living cell, multicellular organism, or in vitro. PCAs can be used

A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.