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CHA00011A

EUR-2019-nCoV E for detection of SARS-CoV-2 RNA

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About This Item

form

solution (in 10 mM Tris, 1 mM EDTA)

packaging

pkg of 1 vial

pH range

7.5-8.5

shipped in

ambient

storage temp.

−20°C

General description

EUR-2019-nCoV E Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2.

The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.

For additional details, please visit SigmaAldrich.com/ncov.

Features and Benefits

The forward and reverse primers in each tube come with the following features:

  • DNA bases
  • No modifications
  • Desalt purified
  • 8 nmol each


The Dual-Labeled Probe in each tube comes with the following features:

  • DNA bases
  • 6-FAM reporter
  • BXQ (Block All Quencher—SigmaAldrich.com/bxq)
  • HPLC purified
  • 4nmol

Components

The product includes 1 individual tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene.

Assay included is E_Sarbeco.The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM, respectively.

E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG

Legal Information

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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