NS0 Cell Line from murine myeloma

85110503, mouse skin, Lymphoblast

• UNSPSC Code: 41106514

Properties

• product name: NS0 Cell Line from murine myeloma, Mouse myeloma, 85110503
• biological source: mouse skin
• form: cell suspension
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse myeloma

Cell Line Description

NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1 ECACC catalogue no. 85011427). The cells are resistant to 10μM azaguanine, cells die in the presence of HAT medium. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

Application

Fusion partner

NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.

Culture Medium

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.