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Key Documents

11-4

11033102

Synonym(s):

11-4

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About This Item

UNSPSC Code:
41106514

biological source

human cervix

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Related Categories

Cell Line Origin

HeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoter

Cell Line Description

11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the HSV-thymidine kinase promoter. 11-4 can be used to evaluate the non-specific transcriptional activity of chemicals.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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