General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.
Application
Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of adult skin (different locations) from pooled donors. They are the major cell type in the epidermis, making up about 90% of the cells. Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.
Quality
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Physical form
Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.
Subculture Routine
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here for more information.
Other Notes
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
Legal Information
RNAlater is a registered trademark of Ambion, Inc.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
