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A6582

Sigma-Aldrich

Antifoam A concentrate

active silicone polymer 100%

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About This Item

UNSPSC Code:
12161901
NACRES:
NA.85

Quality Level

200

form

emulsion (aqueous)

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

density

0.97 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

Related Categories

General description

Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.

Application

Antifoam A concentrate has been used:
  • in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
  • to prevent foam formation during the aerobic treatment of sludge.
  • to study the impact of surface tension on bioaerosol generation during bubbling.

Features and Benefits

  • Molecular biology grade and tested for use in bacterial fermentation.
  • Extremely effective foam suppressor for aqueous and non-aqueous systems.
  • Made of 100% active silicone polymer, free of emulsifiers.
  • Typically, effective at 1-100 ppm.
  • Product will be stable in the pH range of 5 to 9.
  • Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Components

Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .

Preparation Note

Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H410

Precautionary Statements

P273 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

213.8 °F - closed cup

Flash Point(C)

> 101 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M Schlömann et al.
Journal of bacteriology, 172(9), 5119-5129 (1990-09-01)
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. This compound was shown to
F P Ernani et al.
Infection and immunity, 61(6), 2596-2601 (1993-06-01)
Substantial evidence indicates that molecules released by infectious organisms affect virulence and influence immunity to infection. The characterization of extracellular molecules and their mechanism of release is therefore critical to understanding host-parasite relationships. The cestode parasite Mesocestoides corti is known
Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents
T. F. H. G. Jackson, C. D. Dettman, et al.
Laboratory Animal Science, 16, 65-67 (1982)
Factors affecting staphylococcal enterotoxin C bovine production in milk
Hunt K, et al.
International dairy journal, 39(1), 41-46 (2014)
Sarah J Routledge et al.
Microbial cell factories, 10, 17-17 (2011-03-24)
Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactors.
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