A6106

Aprotinin from bovine lung

BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

• Synonym(s): BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
• Empirical Formula (Hill Notation): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• CAS Number: 9087-70-1
• Molecular Weight: 6511.44
• EC Number: 232-994-9
• MDL number: MFCD00130541
• UNSPSC Code: 12352204
• NACRES: NA.77

Properties

• biological source: bovine lung
• Quality Level: 300
• product line: BioUltra
• Assay: ≥98% (SDS-PAGE)
• form: powder
• specific activity: 3-8 TIU/mg solid
• mol wt: ~6,500
• technique(s): inhibition assay: suitable
• solubility: H₂O: 5 mg/mL, clear to very slightly hazy
• UniProt accession no.: P00974
• storage temp.: 2-8°C
• InChI key: ZPNFWUPYTFPOJU-UHFFFAOYSA-N
• Gene Information: cow ... PTI(404172)

Description

Application

Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Preparation Note

A further purification of A1153 to yield a product with slightly higher activity and purity

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves