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93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

Synonym(s):

20x SSC

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

product line

BioReagent

form

solution

technique(s)

Southern blotting: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.0±0.2

density

1.16 g/mL at 20 °C

storage temp.

room temp

General description

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Components

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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