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D0531

Sigma-Aldrich

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride

≥90% purity (HPLC), powder

Synonym(s):

DTAF

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About This Item

Empirical Formula (Hill Notation):
C23H12Cl2N4O5 · HCl
CAS Number:
21811-74-5
Molecular Weight:
531.73
MDL number:
MFCD00051407
UNSPSC Code:
12171500
PubChem Substance ID:
24893242
NACRES:
NA.47

product name

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride, ≥90% (HPLC)

Quality Level

200

Assay

≥90% (HPLC)

form

powder

color

yellow to orange

solubility

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

fluorescence

λex 495 nm; λem 516 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

InChI key

CEKSHCAIHGNWBK-UHFFFAOYSA-N

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Application

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCC6912
MKBP7933V
29396AJV
29396AJ
101H5017

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Bruce L Granger
PloS one, 13(1), e0191194-e0191194 (2018-01-13)
Yeast wall protein 1 (Ywp1) is an abundant glycoprotein of the cell wall of the yeast form of Candida albicans, the most prevalent fungal pathogen of humans. Antibodies that bind to the polypeptide backbone of isolated Ywp1 show little binding
A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures
Wang CS, et al.
Carbohydrate Polymers, 175, 484-492 (2017)
A gelation mechanism for gelatin/polysaccharide aqueous mixtures
Wang CS, et al.
Food Hydrocolloids, 79, 462-472 (2018)
Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress.
Reyes-Becerril M, et al.
Aquaculture (Amsterdam, Netherlands), 280, 39-44 (2008)
Effect of live yeast incorporation in compound diet on digestive enzyme activity in sea bass (Dicentrarchus labrax) larvae.
Tovar D, et al.
Aquaculture (Amsterdam, Netherlands), 204, 113-123 (2002)
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