51976

Ethanol (denatured)

≥99.8%, for molecular biology, (absolute alcohol, without additive, A15 o1), BioUltra

• Synonym(s): Ethyl alcohol
• Linear Formula: CH₃CH₂OH
• CAS Number: 64-17-5
• Molecular Weight: 46.07
• Beilstein: 1718733
• EC Number: 200-578-6
• MDL number: MFCD00003568
• UNSPSC Code: 12191601
• PubChem Substance ID: 329757771
• NACRES: NA.25

Properties

• product name: Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o¹)
• grade: for molecular biology
• Quality Level: 200
• vapor density: 1.59 (vs air)
• vapor pressure: 44.6 mmHg ( 20 °C)
• product line: BioUltra
• Assay: ≥99.8%
• form: liquid
• autoignition temp.: 683 °F
• quality: (absolute alcohol, without additive, A15 o¹)
• expl. lim.: 19 %, 60 °F
• impurities: DNases, none detected; KMnO₄-reducing substances, in accordance; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; substances darkened by H₂SO₄, in accordance; ≤0.0004% free alkali (as NH₃); ≤0.001% aldehyde (as CH₃CHO); ≤0.001% formaldehyde (as HCHO); ≤0.001% ketone (as CH₃COCH₃); ≤0.002% free acid (as CH₃COOH); ≤0.1% methanol (CH₃OH); ≤0.2% water
• evapn. residue: ≤0.001%
• refractive index: n20/D 1.3600 (lit.); n20/D 1.362
• bp: 78 °C (lit.)
• mp: −114 °C (lit.)
• solubility: H₂O: miscible; organic solvents: miscible
• density: 0.789 g/mL at 25 °C (lit.)
• cation traces: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.1 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤1 mg/kg; Ni: ≤0.02 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• SMILES string: CCO
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.04; λ: 280 nm A_max: 0.03
• suitability: suitable for molecular biology
• application(s): life science and biopharma
• format: neat
• InChI: 1S/C2H6O/c1-2-3/h3H,2H2,1H3
• InChI key: LFQSCWFLJHTTHZ-UHFFFAOYSA-N

Description

General description

Ethanol, also known as Ethyl Alcohol, is a volatile primary alcohol with a straight-chain structure. It is frequently employed as a biochemical reagent, effectively denaturing proteins from bacteria, fungi, and viruses. Widely utilized in cell biology, molecular biology, and biochemical research, this DNase, RNase, NICKase, and protease-free form of ethanol plays a crucial role in purifying and precipitating biomolecules like nucleic acids (DNA & RNA) and proteins. Additionally, it finds application in histology for preparing staining and destaining reagents, as well as in the dehydration of tissues before embedding.

Application

Ethanol has been used:

• in vehicle-treated controls to perform mouse experiments
• in viability assay along with crystal violet to stain human and mouse high-grade serous ovarian cancer (HGSOC) cells after stimulation
• to prepare 17β-estradiol (E2) to use in cell culture experiments
• in cell cycle analysis for fixing cells drop-wise

Biochem/physiol Actions

Ethanol is a widely used organic chemical in industry and consumer goods. This aliphatic alcohol is mainly used in industry as a solvent and as an intermediate in the production of other chemicals.

Features and Benefits

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Sales restrictions may apply. Suitable for the precipitation of nucleic acids

Footnote

¹Distribution and use of ethyl alcohol in Switzerland require a license from the Swiss Federal Alcohol Administration

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225 - H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 55.4 °F
• Flash Point(C): 13 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves