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Key Documents

ST1042

Sigma-Aldrich

Anti-PKD2 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Protein Kinase D2

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PKD2(5311)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~105 kDa PKD2 protein.
Recognizes the ~105 kDa PKD2 protein in HEK293 cells.
This Anti-PKD2 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of PKD2.

Immunogen

Human
a synthetic peptide corresponding to amino acids near the C-terminus of human PKD2

Application


Immunoblotting (1:500-1:5000)
Immunoprecipitation (1-4 g/mg lysate)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw.

Analysis Note

Positive Control
HEK 293 cells

Other Notes

Rey, O., et al. 2003. Biochem. Biophys. Res. Commun.302, 817.
Sturany, S., et al. 2002. J. Biol. Chem.277, 29431.
Sturany, S., et al. 2001. J. Biol. Chem.276, 3310.
This antibody does not cross-react with PKD1 or PKD3. Antibody should be titrated for optimal use in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jianfen Guo et al.
The Journal of biological chemistry, 286(8), 6500-6509 (2010-12-16)
Protein kinase D (PKD) exists as a family of structurally related enzymes that are activated through similar phosphorylation-dependent mechanisms involving protein kinase C (PKC). While individual PKD isoforms could in theory mediate distinct biological functions, previous studies identify a high
Clara Aicart-Ramos et al.
The Journal of biological chemistry, 291(45), 23516-23531 (2016-09-25)
Protein kinase D (PKD) isoforms are protein kinase C effectors in signaling pathways regulated by diacylglycerol. Important physiological processes (including secretion, immune responses, motility, and transcription) are placed under diacylglycerol control by the distinctive substrate specificity and subcellular distribution of
Tim Eiseler et al.
The Journal of biological chemistry, 291(1), 462-477 (2015-10-29)
Vesicle formation and fission are tightly regulated at the trans-Golgi network (TGN) during constitutive secretion. Two major protein families regulate these processes: members of the adenosyl-ribosylation factor family of small G-proteins (ARFs) and the protein kinase D (PKD) family of

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