MMA205

Millicell^® µ-Migration Assay Kit

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear & lasts for more than 48 hours.

• Synonym(s): microfluidic cellular migration assay kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• species reactivity: rat, mouse, human
• manufacturer/tradename: Millicell^®
• technique(s): cell based assay: suitable; single cell analysis: suitable
• shipped in: dry ice

Description

General description

• Cell migration occurs in a variety of biological processes includingwound healing, embryonic development, and immune response. Because of the importance of cell migration in angiogenesis, oncology, neurology, andespecially immunology, there is an increased interest in understanding theunderlying biological mechanisms at a single cell level.
• Traditional multiwell (Boyden chamber) migration assays can have limitations, such as: (1) gradients are not linear, well-established, or controlled, (2) quantificationof migratory cells is based on an endpoint measurement rather than a dynamicdetermination, and (3) imaging of the cells while they are migrating iscurrently not possible.
• The Millicell µ-Migration Assay Kit overcomes these limitations.
• Its microfluidic, low-volume technology promotes a stable, diffusion-generatedconcentration gradient that is consistently linear and lasts for more than 48 hours. Designed for video microscopy assays, the µ-Migration Slide is made froma plastic with high optical qualities similar to those of glass. At specifictime intervals, images of the observation area can be acquired, allowingreal-time monitoring and quantitative measurements of cell migration.

Features and Benefits

• Live cell tracking of single cell or cell population migration
• Establishes a stable and linear concentration gradient that lasts ≥ 48 hours
• Helps distinguish chemotaxis from random movement
• Allows for multiparametric analysis, including cell directionality and velocity
• Enhanced optical imaging of slow- and fast-migrating cells
• Analyze 3 chemoattractants in parallel for increased throughput and flexibility
• No need to spend time or money on optimization
• Ready to use, no assembly needed

Components

1. Millicell^® µ-Migration Slides: (Part No. CS204500) Four uncoated sterilized slides. Each slide contains 3 chambers.
2. Millicell^® µ-Migration Caps and Plugs: (Part No. CS204501) Forty-eight of each. Twelve caps and twelve plugs for each slide.
3. Human Collagen IV: (Part No. CS204498) One 100 µg vial of 1 mg/mL Human Collagen IV.
4. Acetic Acid Buffer: (Part No. CS204485) One vial containing 1.5 mL of 0.5 M acetic acid.
5. BSA Solution: (Part No. CS204507) One vial containing 100 µL of 30% BSA solution.
6. Beveled pipette tips: Fisherbrand SureOne 200 µL tips, Fisher Cat. No. 02-707-419. Two 96-tip boxes.

Storage and Stability

1. Millicell^® µ-Migration Slides (Part No. CS204500). Store at room temperature.
2. Millicell µ-Migration Caps and Plugs (Part No. CS204501) Store at room temperature.
3. Human Collagen IV (Part No. CS204498) Store at -20ºC.
4. Acetic Acid Buffer (Part No. CS204485) Store at room temperature.
5. BSA Solution (Part No. CS204507) Store at -20ºC.
6. Beveled Pipette Tips. Store at room temperature.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3