MABN291

Anti-IMPDH1 Antibody, clone 4F9.1

clone 4F9.1, from mouse

• Synonym(s): Inosine-5′-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4F9.1, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001096075
• UniProt accession no.: P20839
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IMPDH1(3614)

Description

General description

Inosine-5′-monophosphate dehydrogenase 1 (IMPDH 1) catalyzes the conversion of inosine 5′-phosphate (IMP) to xanthosine 5′-phosphate (XMP), as part of the de novo synthesis of guanine nucleotides. It therefore is crucial in the regulation of cell growth. IMPDH 1 may also have single-stranded nucleic acid-binding activity involving it in RNA and/or DNA metabolism, and is suspected to play a role in the growth progression of some tumors. Defects in IMPDH 1 abnormalities are linked to retinitis pigmentosa type 10 (RP10) and Leber congenital amaurosis type 11 (LCA11). Due to the connection between IMPDH activity and cell proliferation, it has been become a target for cancer and viral chemotherapy and immunosuppressive drugs. The activities of the antitumor drug tiazofurin, the antiviral drug ribavirin, and the immunosuppressive drugs mizoribine and mycophenolic acid (MPA) are attributed to the inhibition of IMPDH. In addition, bacterial and parasitic IMPDH′s differ significantly from mammalian enzymes, which make it a suitable target for anti-infective drugs.

Immunogen

GST-tagged recombinant protein corresponding to human IMPDH1.

Application

Anti-IMPDH1 Antibody, clone 4F9.1 is a highly specific mouse monoclonal antibody, that targets Inosine-5′-monophosphate dehydrogenase 1 & has been tested in western blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected IMPDH1 in 10 µg of NIH3T3 cell lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected IMPDH1 in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected IMPDH1 in rat eye tissue.

Quality

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected IMPDH1 in 10 µg of PC-12 cell lysate.

Target description

~55 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
PC-12 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable