MAB8126
Anti-Cytomegalovirus Antibody, late Antigen, clone 2D4.2
clone 2D4.2, Chemicon®, from mouse
Synonym(s):
CMV
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2D4.2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2a
shipped in
wet ice
Specificity
Reacts with a late protein M.W. 47-55 kD. Can detect CMV infection 24 hours post-infection exhibiting a cytoplasmic staining which reduces peak intensity at >72 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Immunogen
Epitope: late antigen
Sucrose gradient purified CMV AD169 (ATCC).
Application
Anti-Cytomegalovirus Antibody, late antigen, clone 2D4.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH(P).
IFA at 1:400-1:800 on acetone fixed cells.
Works on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Works on paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH=7.6, 0.1% Sodium Azide.
Storage and Stability
Maintain at +2-8°C for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Development of models and detection methods for different forms of cytomegalovirus for the evaluation of viral inactivation agents.
Vasudevacharya Jayarama, Jennifer Marcello, Asa Ohagen, Veronica Gibaja et al.
Transfusion null
Sylwia Libard et al.
PloS one, 9(9), e108861-e108861 (2014-10-01)
Human cytomegalovirus (HCMV) has been indicated being a significant oncomodulator. Recent reports have suggested that an antiviral treatment alters the outcome of a glioblastoma. We analysed the performance of commercial HCMV-antibodies applying the immunohistochemical (IHC) methods on brain sample obtained
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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