MAB1522
Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7
ascites fluid, clone CGA7, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
clone
CGA7, monoclonal
species reactivity
human, rat, chicken, rabbit, monkey
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
shipped in
dry ice
target post-translational modification
unmodified
Specificity
Antibody recognizes actin isotypes alpha and gamma of smooth muscle cells, but not skeletal or cardiac muscle cells. Reactive with myoepithelium, but nonreactive with other mesenchymal and all epithelial cells. The antibody labels eiomyosarcomas and leiomyomas but does not react with other neoplasms such as carcinomas or lymphoma.
Immunogen
Chicken gizzard actin
Epitope: smooth muscle gamma & alpha actin
Application
Detect Actin using this Anti-Actin Antibody, smooth muscle γ & α actin, clone CGA7 validated for use in WB, IH, IH(P).
Western Blot: 1:1000 (approximately 43 kDa)
Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)
Optimal dilutions must be determined by the end user.
Immunohistochemistry (paraffin, formalin, deparaffinized methacarn-fixed tissue: 1:10,000 or alcohol fixation)
Optimal dilutions must be determined by the end user.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Kristine H Kurtz et al.
PloS one, 9(4), e94082-e94082 (2014-04-09)
The mechanisms that control phasic and tonic contractions of lymphatic vessels are poorly understood. We hypothesized that rho kinase ROCK, previously shown to increase calcium (Ca2+) sensitivity in vascular smooth muscle, enhances lymphatic contractile activity in a similar fashion. Contractions
Smooth muscle actin expression during rat gut development and induction in fetal skin fibroblastic cells associated with intestinal embryonic epithelium.
Kedinger, M, et al.
Differentiation, 43, 87-97 (1990)
Xin Yao et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 13(1), 161-169 (2007-01-04)
Intratumoral microvascular density (MVD) has been controversial as an indicator of prognosis in clear cell renal cell carcinoma (CCRCC). Classification of the intratumoral blood vessels based on differential expressions of blood vessel markers has not been correlated with patient prognosis
Cultured circular smooth muscle from the rabbit colon.
Kao, H W, et al.
In Vitro Cellular & Developmental Biology, 24, 787-794 (1988)
Endogenous gamma-aminobutyric acid modulates tonic guinea pig airway tone and propofol-induced airway smooth muscle relaxation.
G Gallos, NR Gleason, L Virag, Y Zhang, K Mizuta, RA Whittington, CW Emala
Anesthesiology null
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