MAB13424
Anti-MMP-13 Antibody, clone VIIIA2
clone VIIIA2, Chemicon®, from mouse
Synonym(s):
Collagenase-3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
VIIIA2, monoclonal
species reactivity
mouse, human, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MMP13(4322)
Specificity
It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic
Immunogen
Human recombinant collagenase-3 protein.
Application
Immunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature.
* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecipation: use protein G (2 μg/mg protein lysate)
Optimal working dilutions must be determined by end user.
* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecipation: use protein G (2 μg/mg protein lysate)
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
This Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13.
Physical form
Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Analysis Note
Control
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Role of matrix metalloproteinases in recurrent corneal melting.
Kristyna Brejchova,Petra Liskova,Jitka Cejkova,Katerina Jirsova
Experimental Eye Research null
Bu-Nam Jeon et al.
FEBS letters, 593(18), 2665-2674 (2019-06-22)
Dysregulated matrix metalloproteinase (MMP) gene expression is a major cause of the degradation of lung tissue that is integral to emphysema pathogenesis. Cigarette smoking (CS) increases MMP gene expression, a major contributor to emphysema development. We previously reported that Zbtb7c
Ann M Kennedy et al.
The Journal of clinical investigation, 115(10), 2832-2842 (2005-09-17)
MMPs, which degrade components of the ECM, have roles in embryonic development, tissue repair, cancer, arthritis, and cardiovascular disease. We show that a missense mutation of MMP13 causes the Missouri type of human spondyloepimetaphyseal dysplasia (SEMD(MO)), an autosomal dominant disorder
Takwi Nkyimbeng et al.
PloS one, 8(9), e73279-e73279 (2013-09-12)
Idiopathic pulmonary fibrosis (IPF) is a fatal disease characterized by excessive deposition of extracellular matrix (ECM). We investigated the regulation of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in lung fibrosis. MMP and TIMP expression, collagenolytic activity and collagen content
Kaige Ma et al.
International journal of biological sciences, 19(2), 675-690 (2023-01-13)
Pain is the major reason that patients suffering from osteoarthritis (OA) seek medical care. We found that vascular endothelial growth factors (VEGFs) mediate signaling in OA pain pathways. To determine the specific contributions of VEGFs and their receptors (VEGFRs) to
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